First, you need a good restriction enzyme to cut your gene and the plasmid, then use ligase to join them. But listen, our lab's autoclave is always broken, so sometimes we have to sterilize glassware in a pressure cooker like my appa uses for parboiling rice. That's why I say the most important tool isn't the enzyme—it’s making sure everything is sterile, or your selection on ampicillin plates will just grow a forest of contamination.
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